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The cellular infiltrate consists of neutrophils, eosinophils, and lymphocytes. Further magnification in the inset (bold bordered box) shows the presence of the multiple eosinophils.Full figure and legend (105K)Download Power Point slide (414 KB)We have used this mouse model to determine the critical cellular players involved in allergic skin inflammation. The study of mast cell deficient mice indicated that mast cells are not important for the development of Th2 mediated skin inflammation; however, they regulate IFN expression in the skin (Alenius et al., 2002) This is important given the role of IFN in upregulating Fas expression on keratinocytes, thus targeting them for killing by activated FasL T cells (Trautmann et al., 2000) and given the role of IgE mediated reactions in exacerbating AD (Milgrom, 2002).

Myostatin, a negative regulator of skeletal muscle growth, contributes to the maintenance of appropriate muscle mass during development and in adult life. Loss of myostatin activity in cattle, mice, and humans leads to profound muscle overgrowth, associated with increased size and number of fibers.6,7,8,9 Constitutive absence of myostatin function, such as in myostatin null animals, does not permit evaluation of the role of myostatin in adult life, because the observed increased muscle mass can be a consequence of events taking place during muscle histogenesis or prenatal development. Nevertheless, a conditional gene targeting approach exploiting the Cre lox system demonstrated that postnatal inactivation of the myostatin gene is sufficient to cause a generalized muscular hypertrophy of the same magnitude as that observed in myostatin knockout mice.10 Conversely, systemic expression of myostatin leads to reduced muscle mass and muscle wasting (cachexia).11.
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